《植物生理学报》 2015, 51(5): 649-654

小麦离体表皮上气孔对若干关键生理因子的应答反应

魏琳^1,*, 张卫国^3,*, 任柏林¹, 丁博¹, 王俊斌², 李明¹, 陈帅君¹, 程乔林¹, 田秀平¹, 谢晓东^1,**

天津农学院¹农学与资源环境学院, ²基础科学学院, 天津300384; ³沈阳农业大学生物技术学院, 沈阳110866

通信作者：魏琳；E-mail: xiex@tjau.edu.cn；Tel: 022-23798697

摘　要：

构建了气孔分析箱系统, 对离体小麦下表皮进行钾离子、pH值和ABA浓度处理, 观察拥有亚铃形保卫细胞的气孔如何应答不同生理因子, 并与拥有肾形保卫细胞的拟南芥气孔进行了比较。结果表明, 小麦和拟南芥的气孔开度均在钾离子浓度100 mmol•L^-1时最大, 在0 mmol•L^-1时最小; 小麦在pH值6.2时气孔开度最大, 在pH值5.5时最小, 而拟南芥在pH值5.8时气孔开度最大, 在pH值7.0时最小; ABA处理后, 小麦和拟南芥的气孔开度均随ABA浓度增加而变小。此外, 小麦气孔对上述生理因子的应答幅度明显大于拟南芥, 钾离子导致小麦气孔开度最大值比最小值增加了284.99%, 而拟南芥仅增加35.25%, pH值使得小麦和拟南芥的气孔开度分别增加94.54%和33.30%, 施加ABA后气孔开度则分别减小81.4%和56.78%。

关键词：小麦; 生理因子; 离体表皮条; 保卫细胞类型

收稿：2015-03-16   修定：2015-04-09

资助：天津市高校创新团队培养计划(TD12-5017)、天津市学科领军人才计划(80103)、天津市131人才工程(80302)、天津市高等学校科技发展基金计划项目(20130606)、天津市科委应用基础与前沿技术研究计划项目(14JCYBJC30600)和天津市千人计划项目。 * 共同第一作者。

Stomatal Responses to Several Key Physiological Factors Using Epidermal Strips of Wheat

WEI Lin^1,*, ZHANG Wei-Guo^3,*, REN Bo-Lin¹, DING Bo¹, WANG Jun-Bin², LI Ming¹, CHEN Shuai-Jun¹, CHENG Qiao-Lin¹, TIAN Xiu-Ping¹, XIE Xiao-Dong^1,**

¹College of Agriculture, Environment and Resources, ²College of Basic Sciences, Tianjin Agricultural University, Tianjin 300384, China; ³College of Biotechnology, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: WEI Lin; E-mail: xiex@tjau.edu.cn; Tel: 022-23798697

Abstract:

A stomatal bioassay system has been set up to analyze stomatal responses of detached epidermal strips in wheat with dumbbell-shaped guard cells to K⁺, pH and ABA in comparison with those of Arabidopsis with kidney-shaped guard cells. The results showed that the stomata of both wheat and Arabidopsis open following the application of KCl and approached to the largest level under 100 mmol•L^-1. There were slightly different in terms of stomatal responses to pH. Stomatal aperture of wheat reached to the maximal level in pH 6.2 and the minimal stomatal aperture in pH 5.5 while the values in Arabidopsis were pH 5.8 and pH 7.0, respectively. Stomata of both wheat and Arabidopsis came to close as ABA concentration increased. In addition, alteration range of stomata aperture in wheat was significantly wider than those in Arabidopsis. Compared the maximal stomatal aperture with the minimal, K⁺ caused 284.99% and 35.25% of increase in wheat and Arabidopsis, respectively. Those values were 94.54% and 33.30% of increase for pH while 81.4% and 56.78% of decrease for ABA.

Key words: wheat; physiological factors; detached epidermal strips; guard cell forms